Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses.

We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.

Intense exercise and endurance-training program influence serum kinetics of muscle and cardiac biomarkers in dogs.

Few data exist on the physiological consequences of an incremental exercise test (IET) and training in dogs. Here we evaluated the effect of an endurance-training program (ETP) on the kinetics of the serum biomarkers: creatine kinase (CK), aspartate aminotransferase (AST), myoglobin, cardiac troponin I (cTnI), and creatine kinase isoenzyme MB (CK-MB). Eighteen Beagle dogs were assigned to three groups: sedentary (S), untrained (U), and trained (T). The dogs from the groups T and U underwent IET, and their lactate threshold (LT) was determined. The LT and velocity corresponding to the LT (VLT) was determined by visual inspection. The VLT was utilized to determine the training intensity level. Thus, the dogs from group T underwent an eight-week aerobic conditioning program with the intensity training set to 70-80% of the VLT. Next, dogs from the groups T and U have submitted to IET again. The group S did not undergo IETs or training. The maximal velocity (Vmax) at which the dog achieves in the IET and heart rate were determined. For biomarkers, venous blood samples were collected before the experimental procedure (baseline) and before and 1, 6, 12, 24, 48, and 72 h after the IETs. VLT and Vmax increased in group T (P < .05), indicating an improvement in dogs' aerobic and anaerobic fitness. CK and AST rose (P < .05), peaking 6 h after both IETs and returning to baseline levels after 12-24 h. Levels of cTnI and myoglobin were unaltered. CK-MB peaked 1 h after the IETs and returned to baseline levels after 12 h (P < .05). We concluded that ETP improved the aerobic capacity of the dogs without any skeletal or cardiac muscle injury.